Ankita Nag
Atlanta
Summary
Postdoctoral Researcher with 9 years of expertise in microbiology, molecular biology, and protein engineering. Demonstrated proficiency in designing detailed research protocols and conducting experiments, contributing to a strong track record of timely manuscript preparation. Highly skilled in collaboration with fellows, residents, and students to effectively summarize findings and publish results in reputable journals. Aiming to leverage comprehensive research experience in a new postdoctoral role to further advance scientific knowledge.
Overview
10
10
years of professional experience
Work History
Postdoctoral Researcher
Emory University School of Medicine
Atlanta
05.2022 - Current
Project: Delineate the molecular mechanism of sporulation in the gut pathogen Clostridioides difficile.
- Harnessed molecular biology, genetics, and biochemical approaches to uncover the regulatory role of non-canonical histidine kinase PtpC in C. difficile spore formation.
- Explored effects of lactate on stimulating PtpC-mediated sporulation in C. difficile.
- Prepared the information necessary for drafting grant proposals and academic papers.
- Presented research findings at national and international conferences.
PhD Researcher
Indian Institute of Technology Bombay
Mumbai
01.2015 - 08.2021
Project: Identification and characterization of antibiotic-resistant determinants in S. coelicolor, with a focus on Major Facilitator Superfamily efflux pumps and their regulation.
- Identified and studied novel efflux pumps in S. coelicolor contributing to drug resistance insights.
- Conducted in-depth biochemical analysis to evaluate effects of MarR and TetR on efflux pump regulation.
- Executed adaptive laboratory evolution experiments on S. coelicolor to uncover ciprofloxacin-resistant determinants.
- Created assays, standardized media, and protocols to investigate efflux-mediated resistance in S. coelicolor.
- Published experimental and research findings in scientific publications and journals.
Education
PhD - Microbiology
Indian Institute of Technology Bombay
Mumbai, India08-2021
M.Sc. - Microbiology
Maulana Abul Kalam Azad University of Technology (WBUT)
India09.2013
Skills
- Microbiology: Laboratory maintenance of E coli, M smegmatis, Streptomyces coelicolor, C difficile, C elegans
- Anaerobic chamber maintenance
- Molecular Biology: PCR, quantitative PCR, cloning (Gibson, traditional), gene knockout, complementation, site-directed mutagenesis
- Protein Engineering: Purification from model and non-model bacteria
- Protein phosphorylation
- Protein interaction studies(Surface Plasmon Resonance), immunoprecipitation, Western blot, ELISA
- Analytical methods: HPLC, FPLC
- Biochemical assays: Two-hybrid assay, sugar utilization assay, transporter assay
- Phase-Contrast Microscopy
- Image Processing & Analysis: Image J, Image Lab, Origin, Prism
- Genetic Analysis: Molecular Biology {SnapGene, Benchling}, Geneious Prime, NGS Galaxy, AlphaFold, Perseus Quant, MEGA, BLAST analysis
- Writing/Editing: Microsoft Office, EndNote, LaTeX, Zotero, Mendeley
References
- Shonna M. McBride, Associate Professor, Department of Microbiology and Immunology, Emory University, Atlanta, Georgia, United States shonna.mcbride@emory.edu
- Sarika Mehra, Professor, Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, Maharashtra, India sarika@che.iitb.ac.in
- Ganesh Viswanathan, Professor, Indian Institute of Technology Bombay, Mumbai, Maharashtra, India, ganeshav@iitb.ac.in
Publications
- Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the Mar R regulator SCO4122, bioRxiv, doi.org/10.1101/2024.02.28.577416, 02/28/2024
- Involvement of the SCO3366 efflux pump from S. coelicolor in rifampicin resistance and its regulation by a TetR regulator, Applied Microbiology and Biotechnology, 10.1007/s00253-022-11837-x, 02/23/2022
- A Major Facilitator Superfamily (MFS) Efflux Pump, SCO4121, from Streptomyces coelicolor with Roles in Multidrug Resistance and Oxidative Stress Tolerance and Its Regulation by a MarR Regulator, Applied Environmental Microbiology, 10.1128/AEM.02238-20, 03/11/2021
- Distinct transcriptomic response of S. coelicolor to ciprofloxacin in a nutrient-rich environment, Applied Microbiology and Biotechnology, 10.1007/s00253-018-9398-2, 10/16/2018
Conferences
- International Conference on Gram-Positive Pathogens, Omaha, Nebraska, October 13- October16, 2024 (Poster presentation)
- Postdoctoral Symposium, Department of Microbiology and Immunology, Emory University, October 27, 2023 (Oral Presentation)
- ·World Microbe Forum, ASM Microbe, June 20-June 24, 2021. Virtual platform (Poster presentation)
- Research Scholar Symposium, Indian Institute of Technology Bombay, March 7, 2020 (Oral Presentation)
- Multi-drug Efflux Systems, Gordon Research Conference, Lucca (Barga), Italy, April 27- May 3, 2019 (Poster presentation)
- Multi-drug Efflux Systems, Gordon Research Seminar, Lucca (Barga), Italy, April 27- April 28,2019 (Poster and Oral presentation)
- International Conference on Microbiome Research, Pune, India, November 18- November 23, 2018 (Poster presentation)
Awards
- DST( Department of Science and Technology, India) Inspire fellowship
- Gordon Research Conference (GRC) travel award for attending Multidrug Efflux Systems, 2019
Timeline
Postdoctoral Researcher
Emory University School of Medicine
05.2022 - Current
PhD Researcher
Indian Institute of Technology Bombay
01.2015 - 08.2021
PhD - Microbiology
Indian Institute of Technology Bombay
M.Sc. - Microbiology
Maulana Abul Kalam Azad University of Technology (WBUT)
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