Eshetu Debebe

Hess-Jinnah Laboratory

Dystonia is characterized by involuntary movements resulting from heightened muscular activity. Our study investigates the roles of estrogen and progesterone in mice, examining the effects of these hormones on sex differences and their contributions to dystonia.

Animal Care and Welfare: Provided attentive care for 30 mice, ensuring their well-being and health throughout experimental procedures.

Laboratory Techniques: Conducted weekly PCR tests, RNA Scope in situ hybridization (ISH) assay and Boydens chamber Assay

Surgical Skills: Performed ovariectomies, displaying competence in surgical techniques and ensuring the safety of experimental subjects.

Lab Maintenance and Inventory Management: Proficiently ensured the proper functioning and safety of laboratory equipment, while also managing laboratory supplies and materials. Responsibilities included ordering, organizing, and tracking inventory levels to contribute to efficient lab operations.

Toedebusch Laboratory

• The UC Davis CURE Program is funded by the National Cancer Institute to addresses the pressing need for a diverse cancer research community that reflects the nation's heterogeneity, and is sensitive to the significant disparities in cancer health across diverse populations
• My work specifically focused on investigating the role of OLFML3 in promoting glioma cell tumorigenicity. Using a Boydens Chamber Assay, I measured the effects of rhOLFML3 on murine glioma cell migration, invasion, and proliferation. Additionally, I examined the impact of Olfml3 expression knockdown through siRNA on the migration and differentiation phases of angiogenesis in primary mouse brain endothelial cells. By utilizing Matrigel-based tumor microenvironment models, our research uncovered the profound impact of OLML3 on the progression of glioblastoma, an extremely lethal brain tumor without a known cure.

1. Microglia-derived olfactomedin-like 3 is a potent angiogenic factor in primary mouse brain endothelial cells: A novel target for Glioblastoma: Laila M. Joseph, Ryan G. Toedebusch, Eshetu Debebe, Aurelie H. Bastian, Christopher A. Lucchesi, Shafee Sayed-Quadri, Luke A. Wittenburg, Xinbin Chen, Frederick J. Meyers, Christine M. Toedebusch *: 23 November 2022: International Journal of Molecular Sciences

2. Microglia-derived olfactomedin-like 3 Promotes Pro-tumorigenic Microglial Function and Glioma Cell Malignancy: Ryan G. Toedebusch, Chris A. Lucchesi, Eshetu T. Debebe, Luke A. Wittenburg, Xinbin Chen, Christine M. Toedebusch: 2 December 2022: International Journal of Molecular Sciences

• Health coach sessions with a focus on hypertension and diabetes
• Supported implementation of patient health plan, including nutritional wellness, weight loss programs, and personalized fitness and exercise program
• Used an online Athena EHR to take note of vitals and other relevant information
• Accommodate patients to the waiting room
• Conducted initial patient assessment and analysis, prepared patient charts, and performed pre-admissions processing.
• Maintained records of patients' health progress and compared to health goals.

• Tutor students individually or in groups
• Communicate with students and provide various assistance to improve their college experience
• Planned lessons for allotted time to strengthen weak subjects and build skills
• Tracked learning progress to identify opportunities to enhance tutoring methods and help students achieve their goals
• Taught and assisted students in such areas as chemistry, biology, and mathematics creating tailored lesson plans and study guides on subject matter
• Motivated students towards learning and studying to build self-confidence and reduce fear of failure.

• Worked in the Emergency department to ensure clinic efficiency by maintaining proper clinical operations, inventory and patient care with the collaboration of nurses, doctors and hospital staff
• Trained new volunteers
• Took flowers and other personal items to patients on all floors
• Complied with HIPAA standards to confidentially maintain patient medical information
• Completed paperwork and evaluation forms to document patient satisfaction
• Assessed patient room and care environment to optimize patient comfort by keeping areas free of clutter