Ian Pyne
Atlanta
Summary
I am a 3rd year graduate student at Emory University in the Biochemistry, Cell and Developmental Biology Program. I graduated from Duke University with distinction in Biology by completing an undergraduate thesis on the internalization, trafficking, and recycling of Gs-coupled G Protein-Coupled Receptors in the Lefkowitz Laboratory. I am currently working in the Hall Laboratory studying orphan Gs-coupled G Protein-Coupled Receptors.
Overview
4
4
years of professional experience
Work History
Graduate Student
Hall Lab, Emory University
01.2023 - Current
- Research on mechanisms of activation of orphan Gs-coupled G Protein-Coupled Receptors primarily expressed in the brain using mammalian cell culture and luciferase-based cAMP signaling assays.
- Used CRISPR/Cas9 technology to generate knockout cell lines for use in signaling assays.
Research Technician
Radolf & Caimano Lab, UCONN Medical Center
06.2022 - 08.2022
- Projects related to studying persistence of L. interrogans & B. burgdorferi in the Radolf & Caimano Lab.
- Generation of antibodies against protein of interest in B. burgdorferi.
- Purification of proteins of interest expressed in E. coli by affinity chromatography and size exclusion chromatography.
Student Researcher
Lefkowitz Lab, Duke University
02.2021 - 04.2022
- Research completed for an undergraduate honors thesis towards graduation with distinction in Biology, entitled "G Protein-Coupled Receptors: How the Retromer Protein Complex and Adapter Protein SNX27 Mediate Receptor Recycling".
- Purification of proteins of interest expressed in E. coli by affinity chromatography, ion exchange chromatography, and size exclusion chromatography.
- Assayed binding interactions with pull-downs and isothermal titration calorimetry.
Student Intern
Radolf & Caimano Lab, UCONN Medical Center
06.2021 - 07.2021
- Internship studying the persistence of L. interrogans and B. burgdorferi in the Radolf & Caimano Lab at UCONN Medical Center.
Education
Ph.D. - Biochemistry, Cell And Developmental Biology
Emory University
Atlanta, GABachelor of Science - Biology
Duke University
Durham, NC05.2022
Publications
Sokrat B, Nguyen AH, Thomsen ARB, Huang LY, Kobayashi H, Kahsai AW, Kim J, Ho BX, Ma S, Little Jt, Ehrhart C, Pyne I, Hammond E, Bouvier M. Role of the V2R-betaarrestin-Gbetagamma complex in promoting G protein translocation to endosomes. Commun Biol. 2024;7(1):826. Epub 20240707. doi: 10.1038/s42003-024-06512-y. PubMed PMID: 38972875; PMCID: PMC11228049.
Timeline
Graduate Student
Hall Lab, Emory University
01.2023 - Current
Research Technician
Radolf & Caimano Lab, UCONN Medical Center
06.2022 - 08.2022
Student Intern
Radolf & Caimano Lab, UCONN Medical Center
06.2021 - 07.2021
Student Researcher
Lefkowitz Lab, Duke University
02.2021 - 04.2022
Ph.D. - Biochemistry, Cell And Developmental Biology
Emory University
Bachelor of Science - Biology
Duke University
Similar Profiles
Latonia GlanvilleLatonia Glanville
Phlebotomist\Lab Processor at Winship Lab- Emory HealthcarePhlebotomist\Lab Processor at Winship Lab- Emory Healthcare
Taviana McClendonTaviana McClendon
Sr. Leader Surgical Services Specialty Director at Emory University Hospital & Winship Emory MidtownSr. Leader Surgical Services Specialty Director at Emory University Hospital & Winship Emory Midtown
Shorena MeunargiaShorena Meunargia
Lecturer at Tbilisi State Medical University and Emory University School of Medicine partnership program in GeorgiaLecturer at Tbilisi State Medical University and Emory University School of Medicine partnership program in Georgia
Iniobong InyangIniobong Inyang
Volunteer, Social Care Department at Giving HealthVolunteer, Social Care Department at Giving Health
Travis MayTravis May
Customer Service Rep I Remote at HomeCare DeliveredCustomer Service Rep I Remote at HomeCare Delivered