TRISHAALA S

Topic: Gene Therapy using aptamers

• Proficient in isolating plasmids from bacterial cultures
• Experienced in performing restriction enzyme digestion and ligation to clone DNA fragments
• Agarose Gel Electrophoresis
• Nanodrop for quantifying nucleic acids and assessing purity
• MTT Assay
• HDOCK for protein-protein and protein-ligand docking studies

Topic: Embryonic Ethanol Exposure Induced Oxidative and Inflammatory Response in Zebrafish Model

• Zebrafish embryos Model Handling
• Assessed the impact of embryonic ethanol exposure on zebrafish development, growth, and survival rates
• Investigated the potential developmental abnormalities/malformations resulting from embryonic ethanol exposure in zebrafish embryos through microscope
• Performing biochemical assays to measure oxidative stress markers (e.g., ROS, antioxidant levels)

Topic: In-silico vaccine design against Vibrio anguillarum in sea bass using an immunoinformatics approach

• Proficiency in tools like BLAST, Clustal Omega,sequence alignment and phylogenetic analysis
• Experience with software such as PyMOL for protein structure visualization and modeling
• Understanding of immunological principles related to T-cell and B-cell responses
• Familiarity with tools for epitope prediction e.g., IEDB Analysis Resource
• Molecular Docking and Ramachandran Plot Analysis

Department: Department of Pathology, Cytogenetics Lab

• Reagents preparation, sample collection and culture set up, slide preparation, and G-Banding
• Karyotyping techniques
• FISH and IHC